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| 产品名称： | SPI-801 |
|---|---|
| 商品货号： | TS258929 |
| <strong>Cell line </strong>： | SPI-801 |
| DSMZ no. ： | TS258929 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP later by STR fingerprinting |
| Cell type ： | chronic myeloid leukemia in blast crisis (derivative of K-562) |
| Origin ： | allegedly derived "from the peripheral blood of a 10-year-old girl with T-ALL in relapse in 1980"; but DNA fingerprinting showed cross-contamination with K-562; K-562 was established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; SPI-801 cells express and produce hemoglobins (quantitatively and qualitatively different from those of K-562 cells) |
| Reference(s) ： | 14560, 14719, 14720, 15224 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, large, single cells in suspension; image; image; image; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hrs |
| Harvest ： | maximal density at about 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | CD3 -, CD19 -, CD71 +, CD235a +; image |
| Fingerprint ： | same DNA profile as K-562 using multiplex PCR of minisatellite markers |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |