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| 产品名称： | THB-5 |
|---|---|
| 商品货号： | TS258933 |
| <strong>Cell line </strong>： | THB-5 |
| DSMZ no. ： | TS258933 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-C3d receptor and anti-EBV receptor) |
| Origin ： | established by fusion of the P3X63AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with human B cell line SB; cells were reported to produce a monoclonal antibody (IgG2akappa) reacting with the human C3d receptor (CD21) and an EBV receptor of 145 kDa |
| Reference(s) ： | 15056 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension ; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | optimal density at 0.5 x 106 cells/ml, minimal density at 0.1 x 106 cells/ml; split 1:3 to 1:5; after thawing, it is advised to use feeder cells for the first 7-10 days; seed out at ca. 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | maximal density at 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG2a monoclonal antibody |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |