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| 产品名称： | GDM-1 |
|---|---|
| 商品货号： | TS258934 |
| <strong>Cell line </strong>： | GDM-1 |
| DSMZ no. ： | TS258934 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | acute myelomonocytic leukemia |
| Origin ： | established in 1979 from the peripheral blood of a 66-year-old woman with a Philadelphia chromosome negative myeloproliferative disorder (resembling chronic myeloid leukemia, CML, in blast crisis) after transformation to refractory acute myelomonocytic leukemia (AML) |
| Reference(s) ： | 14378 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round to oval cells in suspension; image; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-0.8 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximum density at 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 -, CD14 -, CD15 (+), CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 48(46-48)<2n>XX, +8, +13, -16, +mar, t(2;11)(q36;q13), del(6)(q21), t(7;?)(q35;?), del(12)(p13) |
| Molec. Genetics ： | expression of MNX1 (HLXB9) confirmed by RT-PCR and Western blot |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |