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| 产品名称： | HEP-3B |
|---|---|
| 商品货号： | TS258941 |
| <strong>Cell line </strong>： | HEP-3B |
| DSMZ no. ： | TS258941 |
| Species ： | orginally confirmed as human with IEF of AST, MDH, PEP B and later by STR fingerprinting |
| Cell type ： | hepatocellular carcinoma |
| Origin ： | established from the tumor tissue of an 8-year-old black boy in 1976; description: cells contain a 2.3 kb integrated hepatitis B virus genome fragment; cells were described in the literature to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others; patented cell line |
| Reference(s) ： | 14347, 14673 |
| Biosafety level ： | 1 |
| Risk assessment ： | the cell line contains parts of the hepatitis B virus (HBV) genome integrated into the eukaryotic genome. The HBV surface antigen (HBsAg) is expressed, but no active viruses are produced by the cells. The cells can thus be handled under biosafety level 1. According to the German Commission for Biosafety (ZKBS) the cells can also be handled under biosafety level 1 when the cells are used for genetic modifications. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayers ; image; image; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid stemline with near tetraploid side line (6%) - 63 (60-64)<3n>XX, -Y, -1, -1, -2, -3, -4, -8, -9, -13, +der(11)t(1;11)(q11;q14)x2, der(?15)t(1;?15)(q11;q24), i(7p) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV + (see risk assessment), HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |