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| 产品名称： | A-431 |
|---|---|
| 商品货号： | TS258942 |
| <strong>Cell line </strong>： | A-431 |
| DSMZ no. ： | TS258942 |
| Species ： | originally confirmed as human with IEF of AST, MDH, PEP B and recently by STR fingerprinting |
| Cell type ： | epidermoid carcinoma |
| Origin ： | established from the solid tumor of an 85-year-old woman; cells were reported to have large numbers of EGF binding sites; cell line was used as indicator cell line for anti-TGF binding |
| Reference(s) ： | 14556 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, adherent cell line growing in monolayers; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:4 to 1:6 every 4-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 80-100 hours |
| Harvest ： | about 7-12 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 24% polyploidy - 64(58-64)<3n>XX/XXX, -2, +3, +7, -8, +10, -11, -13, -14, -15, -16, -18, -22, +2mar - del(3)(p11)x1-2, add(4)(p15), add(6)(p21.3), del(7)(p15), der(7)t(7;11)(p11/13;q11/13)add(11)(q23), add(8)(p21), add(11)(q23), der(14)t(13;14)(q11;p11), add(16)(p13) - carries t(7;11) with breakpoint at EGFR (which it overexpresses) - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |