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| 产品名称： | SPI-802 |
|---|---|
| 商品货号： | TS258944 |
| <strong>Cell line </strong>： | SPI-802 |
| DSMZ no. ： | TS258944 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | chronic myeloid leukemia in blast crisis (derivative of K-562) |
| Origin ： | allegedly derived "from the peripheral blood of a 10-year-old girl with T-ALL in relapse in 1980"; but DNA fingerprinting showed cross-contamination with K-562; K-562 was established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; SPI-802 cells express and produce hemoglobins (quantitatively and qualitatively different from those of K-562 cells) |
| Reference(s) ： | 14560, 14719, 14720, 15224 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, large, single cells in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximal density at about 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | CD3 -, CD13 +, CD19 -, CD33 +, CD71 +, CD235a +, HLAclassI -; image |
| Fingerprint ： | same DNA profile as K-562 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypotriploid karyotype without sharp mode - 60-66<3n>XX, -X, -4, -5, -8, -10, -13, -16, -20, -21, -22, -22, +5mar, der(3)t(3;?)(q22;?), del(4)(q22), del(6)(q15)x2, der(2q11q)t(2;11)(2p11p;2q11q), der(15)t(15;18)(q21;q11/12), der(17)t(17;?)(p11/13;?), der(18)t(18;?)(p11;?), del(20)(q11/12), del(X)(p22) - results of FISH analysis show that one or two marker chromosomes probably derive from Ph |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |