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| 产品名称： | NS20Y |
|---|---|
| 商品货号： | TS258948 |
| <strong>Cell line </strong>： | NS20Y |
| DSMZ no. ： | TS258948 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | neuroblastoma |
| Origin ： | established from a A/J mouse strain with neuroblastoma C1300; cells were described in the literature to be aneuploid and to contain high specific activity of choline acetyltransferase |
| Reference(s) ： | 14354 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers or small clumps, round cell body with cellular processes; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split semi-confluent culture 1:5 to 1:10 using trypsin/EDTA (cells can also be detached by tapping the flask); seed out at ca. 2 x 106 cells/80 cm2; viability might decrease to 50-70%; maximal density at ca. 5-10 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | cell harvest of about 1 x 107 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |