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| 产品名称： | YAC-1 |
|---|---|
| 商品货号： | TS258951 |
| <strong>Cell line </strong>： | YAC-1 |
| DSMZ no. ： | TS258951 |
| Species ： | confirmed as mouse by species PCR and as Mus musculus origin by COI DNA barcoding |
| Cell type ： | lymphoma |
| Origin ： | established by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse; cells were described in the literature to be sensitive to the cytotoxic activity of natural killer (NK) cells and are therefore often used as target cells in NK assays |
| Reference(s) ： | 14660 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to oval, single or clustered cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 5 x 105 cells/ml, maintain at 0.3-2.0 x 106 cells/ml; cells grow very fast; split ratio of 1:5 to 1:10 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | maximal density of about 3.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hyperdiploid with 7% polyploidy - 41(38-42)<2n> - mouse chromosome morphology confirmed |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |