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| 产品名称： | TMM |
|---|---|
| 商品货号： | TS258954 |
| <strong>Cell line </strong>： | TMM |
| DSMZ no. ： | TS258954 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | B lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis) |
| Origin ： | established from the peripheral blood of a 62-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) in blast crisis in 1985; cells were described in the literature to be Ph-negative, BCR-ABL1-negative; cells appear to be rather a "normal" B-lymphoblastoid cell line (B-LCL) |
| Reference(s) ： | 14754 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polygonal cells, single or in clusters in suspension; image; image; image; image |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS |
| Subculture ： | seed out at 0.7 x 106 cells/ml; maintain at 0.2-0.8 x 106 cells/ml; split 1:1.5 to 1:2 every 2 to 3 days; the culture usually contains much cell debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-40 hours |
| Harvest ： | saturation density at about 2.5-4.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD33 -, CD37 +, HLA-DR +, anti-EBNA2 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda - |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 8% polyploidy - 46(42-46)<2n>XY - small ring present in 6% cells - no consistent numerical or structural rearrangement - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |