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| 产品名称： | MB-03 |
|---|---|
| 商品货号： | TS258955 |
| <strong>Cell line </strong>： | MB-03 |
| DSMZ no. ： | TS258955 |
| Species ： | Mamestra brassicae origin confirmed by COI DNA barcoding |
| Cell type ： | larval hemocytes |
| Origin ： | established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (cabbage moth; Kohleule) |
| Reference(s) ： | 14712 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic round cells, partly adherent ; image; image |
| Medium ： | 90% Graces Insect (Modified) Medium + 10% h.i. FBS |
| Subculture ： | split cultures 1:2 to 1:3 every 3-5 days (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20 °C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4 °C for up to one week without loss of viability |
| Incubation ： | at 20 °C without CO2 (at room temperature, keep lid of lask closed) |
| Harvest ： | about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |