微信小李
微信小章
产品简介
购买须知
| 产品名称： | B95-8 |
|---|---|
| 商品货号： | TS258958 |
| <strong>Cell line </strong>： | B95-8 |
| DSMZ no. ： | TS258958 |
| Species ： | Sanguinus oedipus origin confirmed by COI DNA barcoding |
| Cell type ： | lymphocytes |
| Origin ： | established from peripheral blood lymphocytes of a cotton-top tamarin (cotton-top marmoset) monkey (Saguinus oedipus); cells were described in the literature to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines |
| Reference(s) ： | 14764, 14765 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B, E |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:5 every 72 hours; minimal density at 0.2 x 106 cells/ml; adherent cells might be detached by tapping the flask; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 41 hours |
| Harvest ： | maximal density at about 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Cytogenetics ： | near-diploid simian karyotype with 4% polyploidy - 44(42-47)<2n> - chromosome rearrangements present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV - |