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| 产品名称： | N4TG3 |
|---|---|
| 商品货号： | TS258962 |
| <strong>Cell line </strong>： | N4TG3 |
| DSMZ no. ： | TS258962 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | neuroblastoma |
| Origin ： | 6-thioguanine resistant mutant of cell line N4 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form increased levels of cAMP in response to prostaglandin E1 which is inhibited by opiate receptors; cells are aneuploid (modal chromosome number of 86) |
| Reference(s) ： | 14355, 15020, 14355 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | flat cell body, slightly adherent, occasionally with short processes; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 100 µM 6-thioguanine |
| Subculture ： | split saturated culture 1:2 to 1:4 every 2-4 days (cells grow slightly adherent, but can be detached without trypsin) |
| Incubation ： | at 37 °C with 10% CO2 |
| Harvest ： | about 10-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Viruses ： | ELISA: reverse transcriptase positive |