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| 产品名称： | N18TG2 |
|---|---|
| 商品货号： | TS258963 |
| <strong>Cell line </strong>： | N18TG2 |
| DSMZ no. ： | TS258963 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | neuroblastoma |
| Origin ： | 6-thioguanine resistant mutant of cell line N18 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form cAMP in response to prostaglandin E1 and to be aneuploid (modal chromosome number of 81) |
| Reference(s) ： | 14598, 14766, 14355 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single, slightly adherent cells, occasionally with short processes |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 100 µM 6-thioguanine |
| Subculture ： | split culture 1:5 to 1:10 every 3-5 days (cells grow adherent, but can be detached without trypsin); seed out at 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | about 1.5 x 107 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |