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| 产品名称： | M-07e |
|---|---|
| 商品货号： | TS258964 |
| <strong>Cell line </strong>： | M-07e |
| DSMZ no. ： | TS258964 |
| Species ： | confirmed originally as human with IEF of AST, MDH, later by STR-typing |
| Cell type ： | acute megakaryoblastic leukemia |
| Origin ： | established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14365, 14366, 14482, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells in suspension, a few cells may be spindle-like and slightly adherent; image; image; image |
| Medium ： | 80-90% Iscoves MDM or RPMI 1640 + 10-20% h.i. FBS + IL-3 (10 ng/ml) or GM-CSF (10 ng/ml) or 10-20% vol conditioned medium of cell line 5637 (DSM ACC 35) |
| Subculture ： | seed out at about 0.5 x 106 cells/ml and maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:3 every two days; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | cell harvest at best at ca. 1 x 106 cells/ml; maximal density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |