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| 产品名称： | OCI-AML2 |
|---|---|
| 商品货号： | TS258966 |
| <strong>Cell line </strong>： | OCI-AML2 |
| DSMZ no. ： | TS258966 |
| Species ： | originally confirmed as human with IEF of MDH, NP and recently by STR typing |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 65-year-old man with acute myeloid leukemia (AML FAB M4) in 1986 at diagnosis; cells were described in the literature to express the retinoic acid receptor gene; cell line carries the DNMT3A R635W mutation |
| Reference(s) ： | 15050, 16667 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells in suspension; image; image; image |
| Medium ： | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.7-1 x 106 cells/ml; it might be of advantage to start culture initially in a 12- or 24-well-plate; after thawing viability decrease to about 50%; possibly no cell growth during the first 3-4 days (do not dilute); later on maintain at 0.7-1.5 x 106 cells/ml and split growing culture 1:2 to 1:3 once or twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | maximal density at about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 3.8% polyploidy - 48(43-49)<2n>XY, +6, +8, der(1)inv(1)(p36q31)t(1;6) (q13;p12), der(2)t(2;17)(p23;q24.1)del(2)(q14.2q36), der(3)t(1;3)(p36;p25), ins(3;2)(q21;q14.2q36), t(5;8)(q11.2;q24), der(6)t(1;6)(q31;p12)t(3;6) (q26;q24), inv(12)(p13.3q13.2), t(13;14)(q32/33;q24.2), der(17)t(2;17)(p23;q24.1) - sideline with +der(5) - carries apparent variant translocations involving several ANLL breakpoints: 1p36, 3q21, 3q26 (megakaryocytic abnormalities), 12p13, 17q24 (FAB-M4) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |