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| 产品名称： | GM-7373 |
|---|---|
| 商品货号： | TS258970 |
| <strong>Cell line </strong>： | GM-7373 |
| DSMZ no. ： | TS258970 |
| Species ： | the species human, mouse and rat were excluded with IEF of LDH, NP, PEP B. Confirmed recently as Bos taurus by COI DNA Barcoding |
| Cell type ： | endothelial aorta |
| Origin ： | established from a male bovine fetus at 24-week gestation; vascular endothelium transformed with benzo(a)pyrene in vitro; cell line described as tumorigenic |
| Reference(s) ： | 14580 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow as adherent multilayer; image; image |
| Medium ： | 80% MEM (with Earles salts) + 20% h.i. FBS + non-essential amino acids + MEM vitamins |
| Subculture ： | split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-60 hours |
| Harvest ： | cell harvest of about 0.8 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hypodiploid bovine karyotype with 15% polyploidy - 58(54-60)<2n> - centric fusion and large submetacentric marker - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |