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| 产品名称： | C6-BU-1 |
|---|---|
| 商品货号： | TS258972 |
| <strong>Cell line </strong>： | C6-BU-1 |
| DSMZ no. ： | TS258972 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | brain tumor |
| Origin ： | this clone is a bromodeoxyuridine-resistant mutant derived from cell line C6 (established from a nitrosourea-induced brain tumor in a Wistar-Furth strain rat in 1971) |
| Reference(s) ： | 14598, 14597, 14355, 14380 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle-shaped, relatively small cells, growing adherently in monolayers; image ; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 100 µM 5-bromodeoxyuridine |
| Subculture ： | split confluent cultures 1:4 to 1:10 every 4 days using trypsin/EDTA; let the cells not grow to complete confluence; seed out at ca. 4 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 30 hours |
| Harvest ： | about 2-3 x 107 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |