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| 产品名称： | CHO-K1 |
|---|---|
| 商品货号： | TS258973 |
| <strong>Cell line </strong>： | CHO-K1 |
| DSMZ no. ： | TS258973 |
| Species ： | originally confirmed as Chinese hamster with IEF and later as Cricetulus barabensis (Chinese stripped hamster) origin by COI DNA barcoding |
| Cell type ： | ovary cells |
| Origin ： | subclone from parental CHO cell line that was initiated from an ovary biopsy of an adult Chinese hamster in 1957 |
| Reference(s) ： | 14649, 14860, 14592 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblastoid cells, may be induced to undergo additional differentiation; image; image; image; image |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | seed out about 105 - 106 cells in an 80 cm2 flask; split confluent cultures 1:3 to 1:10 using trypsin/EDTA (once or twice a week) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | about 10 x 106 cells/80 cm2 or 3 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | same Hinf I-(gtg)5 DNA profile as CHO-DHFR |
| Cytogenetics ： | near diploid karyotype with 12% polyploidy - 22(20-23) - exhibits distinctive CHO karyology |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |