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| 产品名称： | A-549 |
|---|---|
| 商品货号： | TS258975 |
| <strong>Cell line </strong>： | A-549 |
| DSMZ no. ： | TS258975 |
| Species ： | originally confirmed as human with IEF of MDH, NP and cytogenetics, later by DNA fingerprint |
| Cell type ： | lung carcinoma |
| Origin ： | established from an explanted lung tumor which was removed from a 58-year-old Caucasian man in 1972; cells were described in the literature to induce tumors in athmyic mice and to synthesize lecithin |
| Reference(s) ： | 14556, 14717 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells, growing adherently as monolayer; image; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:5 to 1:10 every 4-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | about 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 8% polyploidy - 65(59-66)<3n>XXY, -1, -3, -6, +12, -13, -15, -18, -19, -21, -22, +4mar, der(6)t(1;6)(q11;q27), del(11)(q22-23) - presence of large distinctive der(6) marker confirms identity of this cell line |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |