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产品简介
购买须知
| 产品名称： | MH1C1 |
|---|---|
| 商品货号： | TS258976 |
| <strong>Cell line </strong>： | MH1C1 |
| DSMZ no. ： | TS258976 |
| Species ： | Rattus norvegicus origin confirmed by COI DNA barcoding |
| Cell type ： | hepatoma |
| Origin ： | a clonal strain of epithelial cells was derived from a transplantable Morris hepatoma (#7795, Buffalo strain) in 1967; cells were described in the literature to synthesize and to secrete serum albumin and serum complement component C9 |
| Reference(s) ： | 14531, 14890, 14930, 15209 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in big clusters, different cellular forms |
| Medium ： | 85-90% Dulbeccos MEM + 10-15% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:4 about once or twice per week using trypsin/EDTA; seed out at ca. 2 x 106 cells/75 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 33-60 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | near-diploid rat karyotype with 10% polyploidy - 44(40-46)<2n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |