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产品简介
购买须知
| 产品名称： | L138.8A |
|---|---|
| 商品货号： | TS258980 |
| <strong>Cell line </strong>： | L138.8A |
| DSMZ no. ： | TS258980 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | mast cell |
| Origin ： | established from bone marrow mast cells of a female BALB/c mouse in 1984 |
| Reference(s) ： | 14623, 14624 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in suspension; during exponential growth cells aggregate in small clusters; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | cells are initially (after thawing) difficult to grow! after thawing seed at different concentrations in 24-well plates, later maintain at 0.1-1.0 x 106 cells/ml, do not exceed 1 x 106 cells/ml; split 1:4 to 1:10 about 1-2 times per week. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |