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| 产品名称： | GIRARDI HEART C2 |
|---|---|
| 商品货号： | TS258981 |
| <strong>Cell line </strong>： | GIRARDI HEART C2 |
| DSMZ no. ： | TS258981 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | these cells were cloned from the GIRARDI HEART cell line (passage #623) in 1981 and were thought to "represent human heart cells"; however, isoenzyme analysis (Nelson-Rees et al., Science 191: 96-97, 1976) and DNA fingerprinting at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA; cells were described in the literature to be sensitive to various toxins and hormones |
| Reference(s) ： | 15162 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing as monolayers; image; image |
| Medium ： | 45% Medium 199 (with Hanks salts) and 45% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | about 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed same DNA profile as HELA |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |