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| 产品名称： | MCF-7 |
|---|---|
| 商品货号： | TS258984 |
| <strong>Cell line </strong>： | MCF-7 |
| DSMZ no. ： | TS258984 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR fingerprinting |
| Cell type ： | breast adenocarcinoma |
| Origin ： | established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma (after radio- and hormone therapy) in 1970; cells were described of being positive for cytoplasmic estrogen receptors and having the capability to form domes; cells are described to produce mucus |
| Reference(s) ： | 14975, 15191, 15219, 18201 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayers; image; image; image ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS + MEM non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 2-6 days using trypsin/EDTA; cells may grow slowly after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours (range 30-72 hours) |
| Harvest ： | about 40-50 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To make inquiries about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 8% polyploidy - 86(80-87)<4n>XX, -X, -X, -1, -1, +6, +7, -9, -10, -10, -12, -13, -13, +14, -17, -17, -18, -18, -20, -20, -20, -22, +6-8mar, add(X)(q27), add(2)(q35-37)x1-2, add(5)(p15), del(6)(q25)x1-2, add(7)(p11), add(7)(p14;hsr), add(10)(q23), del(11)(q21q23), add(15)(p11), i(15q), add(16)(q24), add(19)(q13), add(19)(p13)x1-2 - corresponds to published karyotype |
| Molec. Genetics ： | expression of fusion gene BCAS4-BCAS3 confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |