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| 产品名称： | XC |
|---|---|
| 商品货号： | TS258989 |
| <strong>Cell line </strong>： | XC |
| DSMZ no. ： | TS258989 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | sarcoma |
| Origin ： | the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses |
| Reference(s) ： | 14997, 14670, 14904 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, small cells growing as monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + non-essential amino acids |
| Subculture ： | split confluent culture 1:4 every 3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | about 1-2 x 107 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |