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| 产品名称： | SF-21 |
|---|---|
| 商品货号： | TS258992 |
| <strong>Cell line </strong>： | SF-21 |
| DSMZ no. ： | TS258992 |
| Species ： | Spodoptera frugiperda origin confirmed by COI DNA barcoding |
| Cell type ： | ovary cells |
| Origin ： | this cell line (full name: IPLB-SF-21-AE) was derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to Baculovirus infection; see also SF-9 (DSM ACC 125) which was derived from SF-21 |
| Reference(s) ： | 15043 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | 90% round cells; 10% spindle-shaped, mostly adherent, a few cells in suspension; image; image |
| Medium ： | 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate) |
| Subculture ： | split culture 1:3 to 1:5 every 4-5 days; cells grow partly adherent, but the use of trypsin is not necessary, detach cells with rubber scraper; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation ： | 25-29 °C without CO2 (keep lid of flask closed); temperature below 23 °C may lead to reduced growth and loss of adherence |
| Doubling time ： | ca. 3 days |
| Harvest ： | about 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | PCR: SMRV - |