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| 产品名称： | IM-9 |
|---|---|
| 商品货号： | TS258997 |
| <strong>Cell line </strong>： | IM-9 |
| DSMZ no. ： | TS258997 |
| Species ： | confirmed as human with IEF of AST, MDH, NP, PEP B |
| Cell type ： | B lymphoblastoid cells (from patient with multiple myeloma) |
| Origin ： | established from the bone marrow of a woman with multiple myeloma in 1967; cells were described in the literature to synthesize IgG and to have receptors for insulin and calcitonin; cells express BCL2 mRNA; cells appear to be rather "EBV+ B-lymphoblastoid cells" than myeloma-derived cells (see also Pellat-Deceunynk et al., Blood 86: 4001-4002, 1995), hence NOT a malignant myeloma cell line |
| Reference(s) ： | 14486, 14525, 15036 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid, single or clumped cells in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 1-2 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days; as the medium becomes quickly acidic (yellow) without a corresponding cell growth, it is advisable to change the supernatant medium every 1-2 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 45 hours |
| Harvest ： | maximal cell density of 2-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | near diploid - 46(44-46)<2n>XX - apparently normal karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |