微信小李
微信小章
产品简介
购买须知
| 产品名称： | SF-9 |
|---|---|
| 商品货号： | TS259001 |
| <strong>Cell line </strong>： | SF-9 |
| DSMZ no. ： | TS259001 |
| Species ： | Spodoptera frugiperda origin confirmed by COI DNA barcoding |
| Cell type ： | ovary cells |
| Origin ： | cloned from the parent cell line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors |
| Reference(s) ： | 14967, 15043 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic round cells, mostly adherent, a few cells in suspension; image; image; image |
| Medium ： | 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate) |
| Subculture ： | split culture 1:2 to 1:3 every 3-5 days (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber policeman); seed out at ca. 2 x 106 cells/80 cm2; immediately after thawing, cell viability may be low |
| Incubation ： | 25-29 °C without CO2 (keep lid of flask closed); temperature below 23 °C may lead to reduced growth and loss of adherence |
| Doubling time ： | ca. 50 hours |
| Harvest ： | about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | PCR: SMRV - |