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| 产品名称： | L-1210 |
|---|---|
| 商品货号： | TS259002 |
| <strong>Cell line </strong>： | L-1210 |
| DSMZ no. ： | TS259002 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | lymphocytic leukemia |
| Origin ： | established from the methylcholanthrene-induced tumor in a DBA strain mouse (subline 212, 8-month-old female) in 1949; the cells were propagated by heterotransplantation into DBA/2 mice; an in-vitro suspension culture was first reported in 1966; cells are used for cytotoxicity studies |
| Reference(s) ： | 15148, 16802, 15214, 15221 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round, lymphoblast-like cells in suspension |
| Medium ： | 90% Dulbeccos MEM + 10% horse serum |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:4 every 1-2 days; seed out at ca. 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypodiploid karyotype - 39(36-42), with about 75% polyploidy - about 77% of cells carry one or more rearrangements/aberrations |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |