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| 产品名称： | CHO-DHFR- |
|---|---|
| 商品货号： | TS259003 |
| <strong>Cell line </strong>： | CHO-DHFR- |
| DSMZ no. ： | TS259003 |
| Species ： | originally confirmed as Chinese hamster with IEF and later as Cricetulus barabensis (Chinese stripped hamster) origin by COI DNA barcoding |
| Cell type ： | ovary cells |
| Origin ： | subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); patented cell line |
| Reference(s) ： | 14860, 15033 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent monolayer |
| Medium ： | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS + 2 mM L-glutamine (in case other media, e.g. Dulbeccos MEM or Iscoves MDM, are used, hypoxanthine and thymidine should be added as supplements) |
| Subculture ： | seed out at ca. 0.5-2.0 x 106 cells/80 cm2; split confluent cultures 1:3 or higher every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 20-24 hours |
| Harvest ： | about 10-20 x 106 cells/80 cm2 or 40-70 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Cytogenetics ： | near diploid hamster karyotype with 20% polyploidy - 21(20-22)<2n> - distinctive CHO morphology |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |