微信小李
微信小章
产品简介
购买须知
| 产品名称： | NALM-6 |
|---|---|
| 商品货号： | TS259009 |
| <strong>Cell line </strong>： | NALM-6 |
| DSMZ no. ： | TS259009 |
| Species ： | confirmed originally as human with IEF of AST, G6PD, MDH, NP, later by STR typing |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1976. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14625, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, small, round cells in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 1.0-2.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 3 days; minimum density of 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | maximal density of 4-6 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD37 -, cyCD79a +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, cyIgM +, smIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype - 46(43-47)<2n>XY, t(5;12)(q33.2;p13.2) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |