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| 产品名称： | NALM-1 |
|---|---|
| 商品货号： | TS259012 |
| <strong>Cell line </strong>： | NALM-1 |
| DSMZ no. ： | TS259012 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the peripheral blood of a 3-year-old girl with chronic myeloid leukemia (CML) in blast crisis in 1975; cells were described in the literature to be TdT-positive and to carry the Philadelphia chromosome t(9;22) leading to BCR-ABL1 fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14769, 14479, 14488, 14768, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, single, round cells in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | cells are difficult to culture! maintain at 1.0-2.0 x 106 cells/ml; seed out at ca. 2 x 106 cells/ml; cells grow initially slowly; it might be of benefit to start culture in 24-well plates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | maximal density at about 3-5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD80 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 5% polyploidy - 45(42-47)<2n>X, -X, der(9)t(9;22)(q34;q12)x1-2, dup(13)(q21), der(22)t(9;22)(q34;q12) - identity confirmed |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |