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| 产品名称： | SCHNEIDER-2 |
|---|---|
| 商品货号： | TS259013 |
| <strong>Cell line </strong>： | SCHNEIDER-2 |
| DSMZ no. ： | TS259013 |
| Species ： | Drosophila melanogaster origin confirmed by COI DNA barcoding |
| Cell type ： | |
| Origin ： | established from the late embryo of a Drosophila melanogaster (fruitfly) in 1972; originally the cells were diploid with 5-10% XY, currently the cell line has only XX cells that are now 60-80% tetraploid; also often termed (incorrectly) S2 |
| Reference(s) ： | 14943 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small adherent cells growing in monolayers; a small number of cells is also in suspension |
| Medium ： | 45% Schneiders Drosophila medium + 45% TC-100 medium + 10% h.i. FBS |
| Subculture ： | maintain at room temperature without gas exchange (keep lid of flask closed); seed out at ca. 1-2 x 106 in 80 cm2 flask; after thawing cells may have a long lag period (3-7 days) before they start to grow; split confluent culture 1:2 to 1:4 every 2-4 days; cells are resistant to trypsination, for propagation use cells in suspension or remove adherent cells with a rubber policeman |
| Incubation ： | at room temperature up to 30 °C without CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | about 15-20 x 106 cells/25 cm2 |
| Storage ： | frozen with 35% Schneider´s Drosophila medium, 35% TC-100 medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | PCR: SMRV - |