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| 产品名称： | RVH-421 |
|---|---|
| 商品货号： | TS259016 |
| <strong>Cell line </strong>： | RVH-421 |
| DSMZ no. ： | TS259016 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the tumor biopsy of an intracranial secondary melanoma in a 28-year-old European man in 1979; it was described that increasing the tyrosine concentration enhances synthesis of melanin |
| Reference(s) ： | 14756 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing in monolayers; image; image; image |
| Medium ： | 90% RPMI 1640 (or Leibovitzs L-15) + 10% h.i. FBS |
| Subculture ： | seed out 0.5-2 x 106 cells/175 cm2; split confluent culture up to 1:10 one to two times per week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-triploid karyotype with 30% polyploidy - 69(60-70)<3n>XXY, -10, +13, -15, +16, -20, +mar, del(6)(q15), add(8)(q24), del(9)(p22), der(9)t(7;9)(q12;q34)x2, add(11)(q24-25), ins(12;abr)(?q13), der(13)(t(6;13)(p13;q33)x2-3, add(19)(p13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |