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| 产品名称： | 8305C |
|---|---|
| 商品货号： | TS259019 |
| <strong>Cell line </strong>： | 8305C |
| DSMZ no. ： | TS259019 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | thyroid carcinoma |
| Origin ： | established from the primary tumor of a 67-year-old woman with primary thyroid carcinoma (undifferentiated carcinoma, composed of spindle, polygonal and giant cells) |
| Reference(s) ： | 14631 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, large spindle-shaped cells growing as monolayer; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at about 0.5-1 x 106 cells/175 cm2; split confluent culture 1:5 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 55-60 hours |
| Harvest ： | about 5-6 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 22% polyploidy - 74(67-76)<3n>XXX, -2, +9, -10, -12, -13, +14, +15, +15, +18, -19, +20, +4mar, 1-20 dm in most cells, der(6)t(6;7)(q22;q36q11.23), add(15)(p11)x2, del(17)(p12)x2 - subclonal rearrangements, e.g. del(2)(p16), del(2)(q22), del(3)(q22), i(4p), t(6p;7q), del(9)(p23) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |