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| 产品名称： | EM-2 |
|---|---|
| 商品货号： | TS259027 |
| <strong>Cell line </strong>： | EM-2 |
| DSMZ no. ： | TS259027 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3 (DSM ACC 134). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14488, 14656, 14883, 15215, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.2-1.0 x 106 cells/ml; split ratio of 1:2 every 2-3 days; seed out at ca. 0.3-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 h |
| Harvest ： | maximum density at about 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | same DNA profile as EM-3 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with hypotetraploid sideline - 74(70-86)<3n>X, -X, -X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar - der(5)t(5;?)(q13-15;?), der(9)t(9;22)(q34;q11), i(17q)x2 - in some cells up to three copies of der(9) are present - the karyotype overlaps that of EM-3 (DSM ACC 134) and corresponds with those reported for both EM-2 and EM-3 |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |