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| 产品名称： | PLB-985 |
|---|---|
| 商品货号： | TS259030 |
| <strong>Cell line </strong>： | PLB-985 |
| DSMZ no. ： | TS259030 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR fingerprinting |
| Cell type ： | acute myeloid leukemia (derivative of HL-60) |
| Origin ： | allegedly "established from the peripheral blood of a 38-year-old woman with acute myeloid leukemia (AML FAB M4) in relapse in 1985"; DNA fingerprinting showed unequivocally that PLB-985 is a subclone of cell line HL-60 |
| Reference(s) ： | 14491, 15024 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; minimum density at 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximal density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -; image |
| Fingerprint ： | same DNA profile as HL-60 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near diploid karyotype with 4% polyploidy - 46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |