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| 产品名称： | SK-HEP-1 |
|---|---|
| 商品货号： | TS259031 |
| <strong>Cell line </strong>： | SK-HEP-1 |
| DSMZ no. ： | TS259031 |
| Species ： | confirmed originally as human with IEF of AST, MDH, PEP B and later by STR profiling |
| Cell type ： | liver adenocarcinoma |
| Origin ： | established from the ascites of a 52-year-old Caucasian man in 1971 |
| Reference(s) ： | 14537 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayer; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast; seed out at ca. 3-4 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | about 15-18 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 9% polyploidy - 62(60-63)<3n>X, -X, -X, -4, +6, +7, -9, +12, -13, -14, -15, -16, -18, der(1;7)(p10;q10), ?der(2;20)(p10;q10), der(6;13)(p10;q10)x2-3, add(12)(p1?), i(14q), add(19)(p13), add(21)(p11) - sideline with add(15)(p11) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |