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| 产品名称： | MN-60 |
|---|---|
| 商品货号： | TS259033 |
| <strong>Cell line </strong>： | MN-60 |
| DSMZ no. ： | TS259033 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | B cell leukemia |
| Origin ： | established from the peripheral blood of a 20-year-old man with acute B cell leukemia (ALL FAB L3) in partial remission in 1981 |
| Reference(s) ： | 14899 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to polygonal cells in suspension; image; image |
| Medium ： | 90% Hams F10 + 10% h.i. FBS |
| Subculture ： | maintain at 0.2-0.5 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-60 hours |
| Harvest ： | maximal density at 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype - 46(45-47)<2n>XY, dup(1)(q21q41), del(6)(q21), t(8;14)(q24;q32), i(13q) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |