微信小李
微信小章
产品简介
购买须知
| 产品名称： | SUP-T1 |
|---|---|
| 商品货号： | TS259034 |
| <strong>Cell line </strong>： | SUP-T1 |
| DSMZ no. ： | TS259034 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | T cell lymphoma |
| Origin ： | established from the pleural effusion of an 8-year-old boy with T-lymphoblastic lymphoma in relapse |
| Reference(s) ： | 14970 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 2-3 days; after thawing the viability may drop to less than 50%, but the cells recover during the following 4-6 days; |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximal density of 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | cyCD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 1.8% polyploidy - 85<4n>XXX/XXX?Y, -8, -8, -9, -12, inv(2)(p22q11)x2, t(2;?20)(p13;?p11), del(4)(q31q35), del(6)(q25)x2, add(7)(q32), add(9)(q34)x2, inv(14)(q11q32)x2 - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |