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| 产品名称： | P3/NSI/1-AG4-1 |
|---|---|
| 商品货号： | TS259039 |
| <strong>Cell line </strong>： | P3/NSI/1-AG4-1 |
| DSMZ no. ： | TS259039 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | myeloma |
| Origin ： | P3/NSI/1-AG4-1 is a non-secreting clone of P3X63AG8 which is derived from P3K, the induced plasmacytoma MOPC-21; cells do not grow in HAT medium, extensively used in cell fusion studies; cells were described in the literature to synthesize kappa chain, but do not secrete free light chain |
| Reference(s) ： | 14681 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, single and in small clusters in suspension; image; image |
| Medium ： | 92.5-95% Iscoves MDM + 5-7.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | maintain at 0.1-0.8 x 106 cells/ml; split 1:3 to 1:10 every 1-3 days; initially after thawing, the viability may be reduced down to ca. 65%, but cells recover after about 1-2 days |
| Incubation ： | at 37 °C with 7.5% CO2 |
| Doubling time ： | 24 hours |
| Harvest ： | maximal density at 1.2-1.4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hypertriploid murine karyotype with 2% polyploidy - 66(62-72)<3n> - 3-4 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |