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| 产品名称： | COLO-320 |
|---|---|
| 商品货号： | TS259040 |
| <strong>Cell line </strong>： | COLO-320 |
| DSMZ no. ： | TS259040 |
| Species ： | confirmed as human with IEF of AST, G6PD, MDH, NP |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the tumor mass of a 55-year-old Caucasian woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described in the literature to produce several hormones, e.g. serotonin, epinephrine; cells express MYC mRNA |
| Reference(s) ： | 14873 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | undifferentiated, round cells in suspension and loosely adherent in monolayers; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every 24-48 hours (trypsin/EDTA is not necessary as cells are only loosely adherent); seed out at ca. 0.2-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 38 hours |
| Harvest ： | maximal density at about 7 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 53(50-54)<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t(1;5) (p11;q11), der(5)t(5;?)(p15;?), der(7)t(7;?)(q22;?), der(9)t(9;?)(p11;?), del(9)(p22), del(12)(p12), i(13q), der(14)t(14;?)(q24;?), der(18)t(18;?)(p11;?) - variable numbers of supernumerary dmins were present in 90% of cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |