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| 产品名称： | SP2/0-AG14 |
|---|---|
| 商品货号： | TS259043 |
| <strong>Cell line </strong>： | SP2/0-AG14 |
| DSMZ no. ： | TS259043 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | myeloma |
| Origin ： | SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 µg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies |
| Reference(s) ： | 14828, 14959 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image |
| Medium ： | 92.5% Iscoves MDM + 7.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml; viability may be low after thawing and during the first few days, but cells recover after about one week |
| Incubation ： | at 37 °C with 7.5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal density at 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |