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| 产品名称： | SK-MEL-30 |
|---|---|
| 商品货号： | TS259051 |
| <strong>Cell line </strong>： | SK-MEL-30 |
| DSMZ no. ： | TS259051 |
| Species ： | originally confirmed as human with IEF of AST, MDH and recently by STR fingerprinting |
| Cell type ： | melanoma |
| Origin ： | derived from tumor tissue (subcutis metastasis) of a 67-year-old Caucasian man with malignant melanoma in 1975 |
| Reference(s) ： | 14420 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out about 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 about 2-3 times per week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | about 6-8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 3.5% polyploidy - 80(69-83)<3n>XXY-Y/XXYY, +1, +1, +2, +2, +3, +7, +10, -11, +12, +17, +18, +20, -21, +2mar, inv(6)(p11;q25), der(7)t(7;14)(p22;q22), der(9)t(9;?21)(p11;q11)x2, 14p+, der(15)t(15;?)(p12;?)x2, der(16)t(16;?)(q23;?), der(18)t(18;?)(p11;?), mar: ?der(19)t(1;19)(q12;q13) - additional subclonal rearrangements of ch : der(1)t(1;?)(p12;?) were present in a few cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |