微信小李
微信小章
产品简介
购买须知
| 产品名称： | KB-V1 |
|---|---|
| 商品货号： | TS259052 |
| <strong>Cell line </strong>： | KB-V1 |
| DSMZ no. ： | TS259052 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 (DSM ACC 158) which itself is a derivative of the 1951 established HELA (DSM ACC 57) (31-year-old black woman); cells express strongly mdr1 mRNA and protein; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111 |
| Reference(s) ： | 14351, 14898, 14953 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS + 200-1000 ng/ml vinblastine |
| Subculture ： | cells are difficult to culture in the presence of vinblastine! split ratio of confluent cultures 1:3 to 1:10; split every 2-4 days, dependent on the vinblastine concentration, using trypsin/EDTA; upon thawing, first grow the cells without vinblastine, then in increasing amounts; seed out at ca. 3 x 106 cells/80 cm2 in 10 ml medium |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of about 6-10 x 106/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 13% polyploidy - 71-77<3n>XX, -X, +1, +3, +5, +5, +5, +7, +7, +8, +8, -9, -11, +12, +15, -16, +17, -18, -19, +21, -22, +mar, der(1;3)(q10;p10), del(3)(q13), der(3;5)(p10;q10), i(5p)x3, del(7)(q10), der(7)add(7)(p1?)t(7;11)(q3?5;q11), add(8)(q12), del(9)(p24), add(9)(q34), add(12)(p13), add(12)(q1?4), add(15)(p11), add(22)(p11)x2 - drug selection maintains variable numbers of dmin visible in 26% cells as well as smaller particulate chromatin in most remaining cells - karyotype closely related to HELA |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |