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| 产品名称： | NEURO-2A |
|---|---|
| 商品货号： | TS259054 |
| <strong>Cell line </strong>： | NEURO-2A |
| DSMZ no. ： | TS259054 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | neuroblastoma |
| Origin ： | established from the spontaneous tumor of a strain A albino mouse |
| Reference(s) ： | 14667, 14826 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image ; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture ： | split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |