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| 产品名称： | RBL-1 |
|---|---|
| 商品货号： | TS259057 |
| <strong>Cell line </strong>： | RBL-1 |
| DSMZ no. ： | TS259057 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | basophilic leukemia |
| Origin ： | this rat basophilic leukemia (RBL) cell line was established from chlorethylamine-treated rats; the cells were described in the literature to show marked variations in differentiation dependent on the growth rate and the cell cycle phases |
| Reference(s) ： | 14500, 14696 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | 1/4 adherent (epithelial-like cells), 3/4 in suspension (small round cells) |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS (cells may also be grown, but less efficiently, with MEM) |
| Subculture ： | maximal split ratio of 1:4; split 1:3 every 2-3 days; cells grow partly adherent, but trypsin is not needed to detach the cells; seed out at ca. 5 x 106 cells/80 cm2 or 0.3-0.5 x 10 6 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | about 46 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Cytogenetics ： | 74(69-76) - rodent marker chromosomes present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |