微信小李
微信小章
产品简介
购买须知
| 产品名称： | BF-34 |
|---|---|
| 商品货号： | TS259062 |
| <strong>Cell line </strong>： | BF-34 |
| DSMZ no. ： | TS259062 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | this hybridoma cell line was described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat neonatal fibers and, to a lower extent, with all rat adult type 2 fibers in immunofluorescence; it reacts specifically with neonatal MHCs (myosin heavy chain) in Western blotting; established in 1984 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles |
| Reference(s) ： | 14401 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal density at about 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine chromosome morphology |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |