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| 产品名称： | JOSK-I |
|---|---|
| 商品货号： | TS259064 |
| <strong>Cell line </strong>： | JOSK-I |
| DSMZ no. ： | TS259064 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | histiocytic lymphoma (derivative of U-937) |
| Origin ： | supposedly "established from the peripheral blood of a 72-year-old woman with acute myelomonocytic leukemia (AML FAB M4) in 1983"; however DSMZ DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-I is clearly a derivative of U-937 |
| Reference(s) ： | 14491, 14815 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to polygonal cells growing in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.2-1.0 x 106 cells/ml and maintain at 0.3-1.0 x 106 cells/ml; split ratio of 1:2 to 1:5 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | saturation density at about 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD68 +, HLA-DR + |
| Fingerprint ： | same DNA profile as U-937 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hyperdiploid karyotype with 3.5% polyploidy - 56(52-56)<2n>Xder(X), +2, +4, +6, +7, +8, +12, -15, +17, +19, +20, +mar, der(X)t(X;3)(q11;p13)t(1;3)(q11;p25), del(1)(p11p22), t(1;5)(p21.1;q23), t(2;18)(p17;q23), del(4)(q22), add(6)(p23), del(7)(q21)/der(7)t(7;18)(q11.23;p11), der(10)t(10;11)(p14;q22.3)/der(10)t(10;11) (p14;q22.3)dup(10)(q25.1), der(11)t(10;11)(p14;q22.3), add(13)(p13), der(17)t(17;18)(p11;q12) - (mar resembles Ph) - carries uncommon 10;11 translocation associated with AML M5 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |