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| 产品名称： | FLK-BLV-044 |
|---|---|
| 商品货号： | TS259065 |
| <strong>Cell line </strong>： | FLK-BLV-044 |
| DSMZ no. ： | TS259065 |
| Species ： | originally confirmed as sheep by species PCR and recently as ovis aries origin by COI DNA barcoding |
| Cell type ： | embryonal kidney cells |
| Origin ： | established from the embryonal kidney cells of an ovine in 1976; cells are susceptible to infection with BLV (bovine leukemia virus) leading to the formation of syncythia |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with bovine leukemia virus (BLV) and produces active viruses. The viruses are not pathogen to humans, but may be pathogen to animals. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large fibroblast-like cells growing as monolayers; image; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS optional: + 850 mg/L NaHCO3 + 120 mg/L sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split confluent culture 1:2 to 1:8 every 3-5 days using trypsin/EDTA; seed out about 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of about 4 x 106/80 cm2 |
| Storage ： | frozen with 82.5% medium, 10% FBS, 7.5% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |