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| 产品名称： | AM-C6SC8 |
|---|---|
| 商品货号： | TS259067 |
| <strong>Cell line </strong>： | AM-C6SC8 |
| DSMZ no. ： | TS259067 |
| Species ： | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type ： | normal kidney cells |
| Origin ： | derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens |
| Reference(s) ： | 15163 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing in monolayers; image; image |
| Medium ： | 80-90% Medium 199 (Hanks salts) + 10-20% h.i. FBS |
| Subculture ： | cells are difficult to culture and grow slowly to very slowly! split confluent culture 1:5 to 1:10 about once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | cell harvest of about 8 x 106 cells/80 cm2 or 1 x 107 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |